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This repository was archived by the owner on Apr 15, 2026. It is now read-only.
This repository was archived by the owner on Apr 15, 2026. It is now read-only.

When parsing fastq file, convert to BAM #14

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@cmatKhan

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It looks like in fastquorum from fgbio, they parse fastq with umi into a bam with tags. In the alignment step, they use samtools fastq to turn the bam back into a fastq file for alignment with bwa mem.

It is worth looking at this -- if the tags added to the fastq file are retained when converting from bam back to fastq back to bam, then it could potentially make the processing more straightforward

https://github.com/nf-core/fastquorum/blob/ea307015cf134358883d48fcd7a86b365a0a8bb2/modules/local/align_bam/main.nf

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