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[DO NOT MERGE] Pseudo PR for first release - #45

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mashehu wants to merge 527 commits into
TEMPLATEfrom
dev
Open

[DO NOT MERGE] Pseudo PR for first release#45
mashehu wants to merge 527 commits into
TEMPLATEfrom
dev

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@mashehu

@mashehu mashehu commented Apr 8, 2026

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Do not merge! This is a PR of dev compared to the TEMPLATE branch for whole-pipeline reviewing purposes.
Changes should be made to dev and this PR should not be merged!

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Warning

Newer version of the nf-core template is available.

Your pipeline is using an old version of the nf-core template: 4.0.2.
Please update your pipeline to the latest version.

For more documentation on how to update your pipeline, please see the nf-core documentation and Synchronisation documentation.

MaximilianStammnitz and others added 15 commits July 25, 2026 16:41
improved false-double variant filtering
min_counts (1) and base_qual (40) were changed in nextflow.config but not in the
schema, which fails the nf-core lint check nextflow_config/config_defaults.

Co-Authored-By: Claude Opus 5 <noreply@anthropic.com>
Co-Authored-By: Claude Opus 5 <noreply@anthropic.com>
Two small robustness fixes, both leaving the estimator's logic unchanged:

* lm() is fitted on the finite rows only. .usable is already computed directly
  above and the guard already tests it, but the fit ran on the full vector, so a
  single -Inf (log of a zero-coverage double) aborted it with 'NA/NaN/Inf in y'.
  R's default na.action=na.omit already drops NA rows, so pre-subsetting gives
  identical coefficients wherever the fit currently succeeds; it only differs
  where lm() errors out today. The distance range is captured beforehand so the
  coverage lookup cannot end up shorter than the distances it is queried with.

* counts_per_cov_corrected is clamped at zero. The existing guard zeroes both
  columns but tests the *rounded* count, so a subtraction landing in (-0.5, 0]
  rounds to 0, skips the guard, and leaves a negative frequency behind. This
  completes the intent already stated in the code comment.

Co-Authored-By: Claude Opus 5 <noreply@anthropic.com>
The nf-test job fails while pulling the multiqc image with
'failed to register layer: no space left on device' (docker exit 125). The
pipeline pulls ~17 container images, three of them heavy bioconductor/R stacks
(mutscan, DiMSum), which overruns the runner disk part-way through.

Reclaim the preinstalled toolchain before anything is downloaded. The RunsOn
default image is ubuntu24-full-x64, so the same Android/.NET/Haskell bloat as a
GitHub-hosted runner is present and removable. The action SHA is the one already
pinned in download_pipeline.yml.

If this proves insufficient, the next lever is a larger runner volume via a
'volume=80gb' label on the nf-test job.
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4 participants